Proteomics beyond trypsin
نویسندگان
چکیده
منابع مشابه
Rice Proteomics and Beyond
Rice is the most widely consumed staple food for both developed as well as developing world, more so for Asia. According to the data of FAOSTAT (2012), rice has the third-highest worldwide production after sugarcane and maize, among all agricultural crops [1]. Developing countries account for 95% of the total rice production, with China and India contributing for nearly half of the world output...
متن کاملQuantitative proteomics reveals the kinetics of trypsin-catalyzed protein digestion.
Trypsin is the popular protease to digest proteins into peptides in shotgun proteomics, but few studies have attempted to systematically investigate the kinetics of trypsin-catalyzed protein digestion in proteome samples. In this study, we applied quantitative proteomics via triplex stable isotope dimethyl labeling to investigate the kinetics of trypsin-catalyzed cleavage. It was found that try...
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The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that...
متن کاملPharmaceutical Advances and Proteomics Researches
Proteomics enables understanding the composition, structure, function and interactions of the entire protein complement of a cell, a tissue, or an organism under exactly defined conditions. Some factors such as stress or drug effects will change the protein pattern and cause the present or absence of a protein or gradual variation in abundances. Changes in the proteome provide a snapshot of the...
متن کاملA Critical Review of Trypsin Digestion for LC-MS Based Proteomics
Proteomics is defined as the large-scale study of proteins in particular for their structures and functions (Anderson and Anderson 1998), and investigations of proteins have become very important since they are the main components of the physiological metabolic pathways in eukaryotic cells. Proteomics increasingly plays an important role in areas like protein interaction studies, biomarker disc...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2015
ISSN: 1742-464X
DOI: 10.1111/febs.13287